Ядерна фізика та енергетика Nuclear Physics and Atomic Energy   ISSN: 1818-331X (Print), 2074-0565 (Online)   Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine   Languages: Ukrainian, English   Periodicity: 4 times per year   Open access peer reviewed journal

Full text (ua)

V. V. Zhuk^*, O. M. Mikheev, L. G. Ovsyannikova

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, Ukraine

^*Corresponding author. E-mail address: vzhukv@gmail.com

Abstract: The effect of X-ray irradiation of pea seeds at a dose of 20 Gy on the endurance of pea plants to UV-C irradiation at a dose of 5 kJ/m² was studied. It was shown that X-ray irradiation of seeds stimulated plant growth, reduced the effect of UV-C on the plants grown, pigment complex of leaves, stimulated its recovery, and accelerated the utilization of excess H₂O₂ in leaves. It was shown that the effect of X-rays on pea seeds significantly reduced the destructive effect of high-energy ultraviolet radiation on pea plants. Irradiation of dry pea seeds with X-rays stimulated defense mechanisms that increased the resistance of plants to UV-C.

Keywords: pea, X-ray irradiation, UV-C, photosynthetic pigments, H₂O₂.

1. N.A. Zulkifli et al. Advances in irradiation technology for plant resistance: A review. Agric. Rev. 45(1) (2024) 25. https://doi.org/10.18805/ag.RF-284

2. M.-A. Esnault, F. Legue, Ch. Chenal. Ionizing radiation: Advances in plant response. Environ. Exp. Bot. 68 (2010) 231. https://doi.org/10.1016/j.envexpbot.2010.01.007

3. S.S. Araujo et al. Physical methods for seed invigoration: Advantages and challenges in seed technology. Front. Plant Sci. 7 (2016) 646. https://doi.org/10.3389/fpls.2016.00646

4. D.A. Sokolova et al. Relationship of radiation-induced genomic instability and antioxidant production in the chamomile plant. Int. J. Radiat. Biol. 99 (2023) 1631. https://doi.org/10.1080/09553002.2023.2188934

5. D. Sokolova et al. Epigenetic factors of the effect of UV-C and X-ray presowing seeds radiation exposure in Matricaria chamomilla L. genotypes. Int. J. Second. Metab. 11(2) (2024) 305. https://doi.org/10.21448/ijsm.1358437

6. V.V. Zhuk et al. Pre-sowing seeds stimulation by UV-C and X-ray irradiation on the content of antioxidants in inflorescence plants genotypes Matricia chammomila L. Nucl. Phys. At. Energy 22 (2021) 182. (Ukr) https://doi.org/10.15407/jnpae2021.02.182

7. L. Urban et al. Understanding the physiological effects of UV-C light and exploiting its agronomic potential before and after harvest. Plant Physiol. Biochem. 105 (2016) 1. https://doi.org/10.1016/j.plaphy.2016.04.004

8. M.F.A. Dawood et al. Mechanistic insight of allantoin in protecting tomato plants against ultraviolet C stress. Plants 10(1) (2021) 11. https://doi.org/10.3390/plants10010011

9. D.A. Sokolova et al. Involvement of UV-C-induced genomic instability in stimulation plant long-term protective reactions. J. Plant Physiol. 293 (2024) 154171. https://doi.org/10.1016/j.jplph.2024.154171

10. D. Sokolova et al. Productivity of medicinal raw materials by different genotypes of Matricia chammomila L. is affected with pre-sowing radiation exposure of seeds. Int. J. Second. Metab. 8(2) (2021) 127. https://doi.org/10.21448/ijsm.889817

11. V. Zhuk et al. Efficiency of pre-sowing seeds by UV-C and X-ray exposure on the accumulation of antioxidants in inflorescence of plants of Matricaria chamomilla L. genotypes. Int. J. Second. Metab. 8(3) (2021) 186. https://doi.org/10.21448/ijsm.889860

12. V.V. Zhuk, A.N. Mikheev, L.G. Ovsyannikova. The effect of UV-C radiation and cytokinin on pea plants. Faktory Eksperymentalnoi Evolucii Organizmiv 34 (2024) 154. (Ukr) https://doi.org/10.7124/FEEO.v34.1633

13. H.K. Lichtenthaler. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol. 148 (1987) 350. https://doi.org/10.1016/0076-6879(87)48036-1

14. L.-M. Chen, C.-H. Kao. Effect of excess copper on rice leaves: evidence for involvement of lipid peroxidation. Bot. Bull. Acad. Sin. 40 (1999) 283. https://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-05.pdf

15. S. Khorobrykh et al. Oxygen and ROS in photosynthesis. Plants 9 (2020) 91. https://doi.org/10.3390/plants9010091

16. S. Neil, R. Desican, J. Hancock. Hydrogen peroxide signaling. Curr. Opin. Plant Biol. 5 (2002) 388. https://doi.org/10.1016/S1369-5266(02)00282-0

17. M. Sierla et al. Reactive oxygen species in the regulation of stomatal movements. Plant Physiol. 171 (2016) 1569. https://doi.org/10.1104/pp.16.00328

18. A. Sharma et al. Photosynthetic response of plants under different abiotic stresses: A review. J. Plant Growth Regul. 39 (2020) 509. https://doi.org/10.1007/s00344-019-10018-x