Nuclear Physics and Atomic Energy

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Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

  Open access peer reviewed journal

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Nucl. Phys. At. Energy 2017, volume 18, issue 2, pages 179-187.
Section: Radiobiology and Radioecology.
Received: 14.02.2017; Accepted: 12.10.2017; Published online: 22.11.2017.
PDF Full text (ua)
https://doi.org/10.15407/jnpae2017.02.179

Diversity of microflora at the fourth destroyed unit of the ChNPP

O. Yu. Pareniuk1,2, K. E. Shavanova1, V. V. Illienko1, I. O. Simutin3, D. O. Samofalova4, V. B. Rybalka5, K. Nanba2, T. Takasi2, I. M. Gudkov1,*

1 National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
2 Institute of Environmental Radioactivity of Fukushima University, Fukushima, Japan
3 Kyiv Taras Shevchenko National University, Kyiv, Ukraine
4 SI “Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine”, Kyiv, Ukraine
5 Institute for Safety Problems of NPP, National Academy of Sciences of Ukraine, Chornobyl, Ukraine

*Corresponding author. E-mail address: ingudkov@ukr.net

Abstract: DNA of the substrate, sampled from six points in the destroyed 4-th power unit of ChNPP, where the dose rate on the microorganisms ranges from 0.008 to 0.12 Gy/h, was analyzed by New Generation Sequencing technology. It was found that the most diverse and stable microbiome occurs in sample, located outside of the "Ukryttya" object on the industrial site (conditional control). There are no dominants in it, which means that it is the most balanced and approximate to the general state of the soil microbiome of ecosystems surrounding the ChNPP. As for the sample, taken from the spot, where the dose rate was the highest, total number of species represented appeared eight times smaller, but dominance index was the highest, which indicates the formation of distinct microbiome dominants.

Keywords: ChNPP, the fourth unit, radionuclide contamination, DNA sequencing, microflora.

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