Nuclear Physics and Atomic Energy

ßäåðíà ô³çèêà òà åíåðãåòèêà
Nuclear Physics and Atomic Energy

  ISSN: 1818-331X (Print), 2074-0565 (Online)
  Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine
  Languages: Ukrainian, English, Russian
  Periodicity: 4 times per year

  Open access peer reviewed journal


 Home page   About 
Nucl. Phys. At. Energy 2016, volume 17, issue 2, pages 166-175.
Section: Radiobiology and Radioecology.
Received: 09.09.2015; Accepted: 29.06.2016; Published online: 10.08.2016.
PDF Full text (ru)
https://doi.org/10.15407/jnpae2016.02.166

Using the cytogenetic dosimetry for the control of potential over exposure of contractors enterprises staff of SSE ChNPP

L. K. Bezdrobna1,*, L. V. Tarasenko1, T. V. Tsyganok1, T. V. Melnyk1, V. A. Kurochkina1, V. O. Sushko2, S. Yu. Nechayv2, L. I. Shvayko2, Î. Î. Kolosynska2

1 Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine
2 SI “National Research Centre for Radiation Medicine MAS of Ukraine” (NRCRM), Kyiv, Ukraine


*Corresponding author. E-mail address: lbezdrob@ukr.net

Abstract: Cytogenetic examination of two groups of contractors staff of SSE ChNPP who performed the work on the preparation of pits for the construction of the new confinement (22 workers), and the dismantling of the “Ukryttya” ventilation pipe (12 workers) was provided. There were revealed three individuals, who had individual frequency of specific markers of radiation (dysenteric chromosomes with accompanying fragments) significantly exceeding the mean population levels and the average rate in the comparison groups of persons who had not occupational contacts with radiation factor. It indicated the probability of their additional background radiation while performing the work in the ChNPP zone. Calculated by dysenteric chromosomes frequency indicative “biological” doses of their irradiation show more significant impact of the radiation factor than that resulted from the data of physical dosimetry.

Keywords: chromosome aberrations, the culture of blood lymphocytes, cytogenetic dosimetry, the staff, the “Ukryttya” object.

References:

1. V.G. Bebeshko, D.A. Bazyka, V.O. Sushko et al. Radiation protection and health of the contractor companies workers performing transformation of the Chornobyl NPP shelter object into the ecologically safe system, based on the clinical and dosimetric registry. Problems of Radiat. Medicine and Radiobiology 14 (2009) 40. (Ukr) http://www.radiationproblems.org.ua/Problemy_rad_med_radiobiol_14_2009.pdf

2. Biological indication of radiation influence on human body using cytogenetic methods. Medical Technology No. ÔÑ-2007/015-Ó (Ìîskva, 2007) 29 p. (Rus)

3. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies (Vienna: International Atomic Energy Agency, 2011) 229 p. Book

4. Rules of Medical Surveying for Personnel of Certain Categories. The Decree of the Ministry of Health of Ukraine No. 246 (May 21, 2007). (Ukr) http://zakon3.rada.gov.ua/laws/show/z0846-07

5. S. Balakrishnan, S.B. Rao. Cytogenetic analysis of peripheral blood lymphocytes of occupational workers exposed to low levels of ionising radiation. Mutat. Res. 442 (1999) 37. https://doi.org/10.1016/S1383-5718(99)00056-X

6. G. Stephan, S. Pressl. Chromosomal aberrations in peripheral lymphocytes from healthy subjects as detected in first cell division. Mutat. Res. 446 (1999) 231. https://doi.org/10.1016/S1383-5718(99)00191-6

7. N.P. Bochkov, A.N. Chebotarov, L.D. Katosova, V.I. Platonova. The Database for Analysis of Quantitative Characteristics of Chromosome Aberration Frequencies in the Culture of Human Peripheral Blood Lymphocytes. Russian Journal of Genetics 37(4) (2001) 440. (Rus) https://doi.org/10.1023/A:1016618929755

8. A.V. Sevan’kaev, I.K. Hvostunov, G.P. Snigiryova et al. Comparative analysis of results on the cytogenetic examination of control groups individuals in different national laboratories. Radiatsionnaya Biologiya. Radioekologiya 53(1) (2013) 5. (Rus) https://doi.org/10.7868/S0869803112060124

9. R.J. Purrot, D.C. Lloyd, G.W. Dolphin et al. The study of chromosome aberration yield in human lymphocytes as indicator of radiation dose: III. A review of cases investigated: 1971 - 1972. NRPB-RIO (1973).

10. N.A. Maznyk, V.A. Vinnikov. Calibration dose-response relationships for cytogenetic biodosimetry of recent and past exposure to low dose gamma-radiation. Ukrainian Journal of Radiology 12 (2004) 415.

11. I.E. Vorobtsova, M.V. Vorob’eva, A.N. Bogomazova et al. Dose dependence of stable and unstable chromosome aberrations in human lymphocytes γ-inradiated in vitro. Radiatsionnaya Biologiya. Radioekologiya 37(2) (1997) 233. (Rus)

12. Biological Dosimetry for Persons Irradiated by the Chernobyl Accident. Eds D.C. Lloyd, A.V. Sevan’kaev. EUR Report No. 16532 (Brussels: CEC, 1996).

13. Y. Suto, M. Hirai, M. Akiyama et al. Biodosimetry of Restoration Workers for the Tokyo Electric Power Company (TEPCO) Fukushima Daiichi Nuclear Power Station Accident. Health Physics 105(4) (2013) 366. https://doi.org/10.1097/HP.0b013e3182995e42

14. V. Martins, A.C. Antunes, O. Monteiro Gil. Implementation of a dose-response curve for γ-radiation in the Portuguese population by use of the chromosomal aberration assay. Mutat. Res. 750 (2013) 50. https://doi.org/10.1016/j.mrgentox.2012.09.009

15. Radiation Safety Standards (RSSU-97) (Kyiv: Ministry of Health of Ukraine, 1997) 121 p. (Ukr) http://www.insc.gov.ua/docs/nrbu97.pdf

16. J. Pohl-Ruling, P. Fescher, O. Haas et al. Effect of low dose acute X-irradiation on the frequencies of chromosomal aberrations in peripheral lymphocytes in vitro. Mutat. Res. 110(1) (1983) 71.

17. D.C. Lloyd, A.A. Edwards, A. Leonard et al. Chromosomal aberrations in human lymphocytes induced in vitro by very low doses of X-rays. Int. J. Radiat. Biol. 61 (1992) 335. http://dx.doi.org/10.1080/09553009214551021

18. Safety report of the “Shelter” object during 2011. VTS 04, 27.01.2012. 50 p. (Ukr)

19. E.A. Aseeva, G.P. Snigiryova, A.L. Neverova et al. The multiabberant cells in groups of people exposed to radiation due to different situations and their possible biological part. Radiatsionnaya Biologiya. Radioekologiya 49(5) (2009) 552. (Rus)

20. A.V. Sevan’kaev, A.F. Tsyb, D.C. Lloyd et al. “Rogue” cells observed in children exposed to radiation from the Chernobyl accident. Int. J. Radiat. Biol. 63 (1993) 361. http://dx.doi.org/10.1080/09553009314550481

21. D.C. Lloyd, J.N. Lucas, A.A. Edwards et al. A study to verify a reported excess of chromosomal aberrations in blood lymphocytes of Namibian uranium miners. Radiat. Res. 155 (2001) 809. https://doi.org/10.1667/0033-7587(2001)155[0809:ASTVAR]2.0.CO;2

22. L.K. Bezdrobnaya, L.V. Tarasenko, T.V. Tsyganok et al. Cytogenetic effects in Chornobyl NPP Object "Shelter" personnel. Proc. 4th Biennial Conference “The health effects of low and very low dose exposure to ionizing radiation” (Hamilton, Ontario, Canada, 2005) p. 43.

23. L.K. Bezdrobnaya, T.V. Tsyganok, E.P. Romanova et al. The dynamics of cytogenetic effects in the blood lymphocytes of people residing without permission in ChNPP exclusion zone. Radiatsionnaya Biologiya. Radioekologiya 42(6) (2002) 727. (Rus)