Ядерна фізика та енергетика Nuclear Physics and Atomic Energy   ISSN: 1818-331X (Print), 2074-0565 (Online)   Publisher: Institute for Nuclear Research of the National Academy of Sciences of Ukraine   Languages: Ukrainian, English   Periodicity: 4 times per year   Open access peer reviewed journal

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N. K. Kutsokon¹, D. M. Grodzinsky¹, N. M. Rashidov¹, V. V. Trishin<sup>2

1</sup>Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine
²Institute for Nuclear Research, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Abstract: The present study was designed to elucidate the rate of chromosome damages induced by ²⁴¹Аm in plant test-system. The seedlings of onion Allium cepa L. were used as a test-system. The impact of ²⁴¹Аm - irradiation on the frequency of chromosome aberration (FCA) in root tip cells (using anaphase method), mitotic index (MI) and energy of germination (EG) was evaluated. The seed were soaked and germinated in ²⁴¹AmCl₃ solutions different concentrations (1.5 · 10^-9 - 3.0 · 10^-7 g/l). Water solution-to-plant transfer factor for ²⁴¹Аm was found to be 0.18 ± 0.04. The effect of γ-irradiation on FCA on the same assay was also evaluated for comparison with that of ²⁴¹Аm to establish the relative biological effectiveness (RBE) of ²⁴¹Аm. We revealed strong effects on both FCA and MI at γ-irradiation. The clearest impact of ²⁴¹Am irradiation on EG-parameter was registered. We had not received statistically significant values that could demonstrate any changes in FCA in seedlings grown on ²⁴¹Am-contained solution in comparison with the control. We revealed the greatest rate of difference of FCA in one case, when concentration of ²⁴¹Am in solution was 1.5 · 10^-8 g/l. This rate was taken to account the RBE and it amounted to 58 ± 18. The real number of RBE should be some lower due to differences between condition of α- and γ-irradiation.

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